The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
Q620 WashLab760 Laboratory Bottle Washer Kimadi
A-105173 Miniature Skull Axis Scientific
4306 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Orange Bio Plas
V1002-8 Optional eight channel adapter, for use with 200ul pipette tips Accuris
710611100-FreeZone 6 Liter -84C Console Freeze Dryer- Labconco
A-105868, Human Skull With Masticatory Muscles Axis Scientific
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
3970400- 4' Purifier Vertical Clean Bench- Labconco
A-105171 Half Disarticulated Human Skeleton Axis Scientific
V1002 Handheld vacuum controller with single channel adapters Accuris
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
700611130- FreeZone 6 Liter -50C Console Freeze Dryer- Labconco
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
710611000-FreeZone 6 Liter -84C Console Freeze Dryer- Labconco
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
RE301B-WV - Rotary evaporator, Glass "B", Water Bath, VR300 - Yamato
850071 Environmental Quality SD Card Logger Sper Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
D4074- Quick-DNA Microprep Plus Kit- Zymo Research 


