The Methylated-DNA IP Kit is designed for enrichment of 5mC-containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis. It features a highly specific Anti-5-Methylcytosine Monoclonal Antibody for the immunoprecipitation of methylated DNA in only a few hours. This kit is capable of achieving over one hundred-fold enrichment of methylated DNA vs. non-methylated DNA. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including PCR, bisulfite treatment, whole-genome amplification, ultra-deep sequencing, and microarray. Control DNA and primers are included to monitor the success of the assay.
| Technical specifications | |||||||
| Applicable For | Immunoprecipitation of methylated DNA, PCR, sequencing, microarrays, etc. | ||||||
| Elution Volume | ≥ 15 µl | ||||||
| Input | 50 – 500 ng of DNA | ||||||
| Processing Time | 4 hours | ||||||
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
RE301B-O - Rotary evaporator, Glass "B", Oil Bath- Yamato
A-105868, Human Skull With Masticatory Muscles Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
28506-QIAquick PCR & Gel Cleanup Kit (100)- Qiagen
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
SF 3300- STAT FAX 3300- Awareness Technology
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
BSWNMR Block, 30 x NMR tubes Benchmark Scientific
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
BSWCMB Quick-Flip Block Benchmark Scientific
A-105168 Miniature Human Skeleton Axis Scientific
A-105172 Human Skeleton With Flexible Spine, Muscle Insertions, And Ligaments Axis Scientific
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
A-105171 Half Disarticulated Human Skeleton Axis Scientific 



