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D5032/D5033- EZ-96 DNA Methylation-Lightning Kit- Zymo Research

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The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit (Cat. D5033), samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc.

Technical specifications
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume Deep-well: ≥ 15 µl
Shallow-well: ≥ 30 µl
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input 100 pg – 2 µg of DNA.
Processing Time 1.5 hours
Recovery > 80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
SKU: D5032/D5033 Categories: , Tags: , Brand:
Product

D5032 (EZ-96 DNA Methylation-Lightning Kit (Shallow-Well) – 2 x 96 Rxns.), D5033 (EZ-96 DNA Methylation-Lightning Kit (Deep-Well) – 2 x 96 Rxns)

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