Kits for Epigenetics, Reagent and Kits
D5032/D5033- EZ-96 DNA Methylation-Lightning Kit- Zymo Research
$487.00
The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit (Cat. D5033), samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc.
| Technical specifications | |||||||
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. | ||||||
| Conversion | > 99.5% | ||||||
| Elution Volume | Deep-well: ≥ 15 µl Shallow-well: ≥ 30 µl |
||||||
| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. | ||||||
| Input | 100 pg – 2 µg of DNA. | ||||||
| Processing Time | 1.5 hours | ||||||
| Recovery | > 80% | ||||||
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. | ||||||
| Product | D5032 (EZ-96 DNA Methylation-Lightning Kit (Shallow-Well) – 2 x 96 Rxns.), D5033 (EZ-96 DNA Methylation-Lightning Kit (Deep-Well) – 2 x 96 Rxns) |
|---|
