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D5030T/D5030/D5031- EZ DNA Methylation-Lightning Kit- Zymo Research

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The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.

Technical specifications
Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume ≥ 10 µl
Equipment Thermocycler with heated lid and microcentrifuge.
Input 100 pg – 2 µg of DNA.
Processing Time 1.5 hours
Recovery > 80%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.
SKU: D5030T/D5030/D5031 Categories: , Tags: , , Brand:
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D5030T (EZ DNA Methylation-Lightning Kit – 10 Rxns), D5030 (EZ DNA Methylation-Lightning Kit – 50 Rxns), D5031 (EZ DNA Methylation-Lightning Kit – 200 Rxns)

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