The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing | ||||||
| Elution Volume | ≥ 20 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into 20 µl | ||||||
				
4301 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Red Bio Plas							
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas							
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas							
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex							
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas							
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific							
4300 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Natural Bio Plas							
FP-313 - High Performance Muffle Furnace 7.5L 220V- Yamato							
A-104405, 3-Part Life-Size Human Skull Axis Scientific							
A-104406 Complete Disarticulated Human Skeleton Axis Scientific							
A-105176 Disarticulated Half Child Skeleton Axis Scientific							
A-105170 Miniature Painted Human Skeleton Axis Scientific							
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas							
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific							
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific							
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas							
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific							
4306 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Orange Bio Plas							
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5)  Bio Plas							
4305 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Amber Bio Plas							
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas							
4304 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Green Bio Plas							
A-105868, Human Skull With Masticatory Muscles Axis Scientific							
A-105172 Human Skeleton With Flexible Spine, Muscle Insertions, And Ligaments Axis Scientific							
A-105173 Miniature Skull Axis Scientific							
12018-2E -S-EVAP-KD  8 position, bath 1600W for 500mL Kuderna-Danish flasks   - Organomation (Copy)							



