$677.00 – $1,009.00
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing | ||||||
| Elution Volume | ≥ 20 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into 20 µl | ||||||
| Product | D4303 (ZymoBIOMICS 96 DNA Kit (Lysis Rack) – 2 x 96 Preps), D4307 (ZymoBIOMICS 96 DNA Kit (No Lysis Matrix) – 2 x 96 Preps), D4309 (ZymoBIOMICS 96 DNA Kit (Lysis Tubes) – 2 x 96 Preps) |
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