The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 37.5 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block | ||||||
| Processing Volume | Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum) | ||||||
0099 Black Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
SF4500- Stat Fax®4500- Awareness Technology
A-105867, Human Skull Model With Flexible Neck Axis Scientific
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
EM-1624 Round Block - 24 Hole, sample tube volume selectable Goojung (Copy)
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
A-105172 Human Skeleton With Flexible Spine, Muscle Insertions, And Ligaments Axis Scientific
0094F Fluorescent Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
12010-2E -S-EVAP-KD 10 position, bath 1600W for 250mL Kuderna Danish flasks - Organomation
A-105171 Half Disarticulated Human Skeleton Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
0090 Natural Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105176 Disarticulated Half Child Skeleton Axis Scientific
WashLab790 Laboratory Bottle Washer Kimadi
A-105170 Miniature Painted Human Skeleton Axis Scientific
A-104405, 3-Part Life-Size Human Skull Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
A-105852 Classic Flexible Human Skeleton Axis Scientific
VV0020-BOT Graduated, PC collection bottle (included) Accuris
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-105168 Miniature Human Skeleton Axis Scientific
840003 Digital Anemometer Thermometer Sper Scientific
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
A-105866 Miniature Skeleton With Flexible Spine Axis Scientific
A-105868, Human Skull With Masticatory Muscles Axis Scientific 


