The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤50 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2×108bacterial, 2×107yeast, or 2×106mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C. | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 5 µg total DNA can be eluted into ≥ 10 µl | ||||||
701222130- FreeZone 12 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
710621100-FreeZone 6 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
710402010-FreeZone 4.5 Liter -84C Benchtop Freeze Dryer- Labconco
720401010-FreeZone 4.5 Liter -105C Benchtop Freeze Dryer- Labconco
701812110-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
710611010-FreeZone 6 Liter -84C Console Freeze Dryer- Labconco
700621030- FreeZone 6 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
711221110-FreeZone 12 Liter -84C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
3970322- 3' Purifier Filtered PCR Enclosure with UV Light and Protection Panel- Labconco
701812070-FreeZone 18 Liter -50C Console Freeze Dryer- Labconco
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0092F Fluorescent Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0091F Assorted Fluorescent Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen 



