The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 50 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109bacterial, 2×108yeast, or 2×107mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 25 µg total DNA can be eluted into ≥50 µl | ||||||
A-105852 Classic Flexible Human Skeleton Axis Scientific
QIAamp DNA Blood Mini Kit (250) - 51106 - Qiagen
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-104405, 3-Part Life-Size Human Skull Axis Scientific
A-105178, 3-Part Life-Size Human Skull With 8-Part Brain Axis Scientific
A-105176 Disarticulated Half Child Skeleton Axis Scientific
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
V0020-F1 Hydrophobic filter, pk/5 ( 1 each included) Accuris
EC-1648S Evatros C(2~16ml Tube, 48 Hole) + Evatros G(60L N2 & 200L Dry Air Switching) Goojung
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
0093 Green Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
9136045 40x Phase Slider for use with P/N 3124041 (LP series) Labomed
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
4307 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
VV0020-BOT Graduated, PC collection bottle (included) Accuris
4306 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Orange Bio Plas
A-105173 Miniature Skull Axis Scientific
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
A-105868, Human Skull With Masticatory Muscles Axis Scientific
V1002 Handheld vacuum controller with single channel adapters Accuris
4305 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Amber Bio Plas
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
A-104271, 3-Part Life-Size Painted Didactic Human Skull Axis Scientific
0095F Fluorescent Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas 

