The ZymoPURE – Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE – Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE – Express Plasmid Midiprep Kit contains patented colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization and syringe filters are included for rapid lysate clearing. No large centrifuge! No cell pelleting! No gravity filtration! No ethanol precipitation! From culture to endotoxin-free plasmid DNA in 15 minutes!
| Technical specifications | |||||||
| Applicable For | Eluted DNA is well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications. | ||||||
| Binding Capacity | 1.2 mg | ||||||
| Culture Input | ≤ 25 mL | ||||||
| Elution Volume | ≥ 200 µl | ||||||
| Endotoxin Levels | ≤1 EU/µg Plasmid DNA | ||||||
| Equipment | Microcentrifuge and vacuum/vacuum manifold | ||||||
| Format | Spin Column | ||||||
| Processing Time | 15 min | ||||||
| Purity | Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0 | ||||||
| Recommended Sample Volume | ≤ 25 ml | ||||||
| Size Range | Up to 200 kb | ||||||
| Yield | ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized). 1.2 mg maximum binding capacity of column. | ||||||
0092 Blue Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104264, 16-Part Mini Torso Axis Scientific
V1002-8 Optional eight channel adapter, for use with 200ul pipette tips Accuris
0094 Orange Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-105176 Disarticulated Half Child Skeleton Axis Scientific
A-105170 Miniature Painted Human Skeleton Axis Scientific
4303 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Blue Bio Plas
4307 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Lavender Bio Plas
4301 Polypropylene 1.5ml Graduated Flat Top Microcentrifuge Tube, Red Bio Plas
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
A-105868, Human Skull With Masticatory Muscles Axis Scientific
A-106193, Didactic Skull On Cervical Vertebrae With Nerves And Arteries Axis Scientific
4000 Blue Polypropylene Snap Cap Microcentrifuge Tube Opening Tool (Case Of 100) Bio Plas
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific 



