The ZymoPURE – Express Plasmid Midiprep Kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of high-quality endotoxin-free plasmid DNA directly from the culture without the pelleting and resuspension steps. Just add the ZP Express Lysis Buffer directly to your bacterial culture, neutralize, filter debris, and purify using the patented Zymo-Spin V-P microcentrifuge column. The ZymoPURE – Express Plasmid Midiprep Kit is the fastest and easiest method for isolating plasmid DNA from E. coli. The innovative binding chemistry (patent pending) enables highly concentrated DNA to be eluted directly from microcentrifuge column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, and protein. The eluted plasmid DNA ideal for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications. As an added convenience, the ZymoPURE – Express Plasmid Midiprep Kit contains patented colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization and syringe filters are included for rapid lysate clearing. No large centrifuge! No cell pelleting! No gravity filtration! No ethanol precipitation! From culture to endotoxin-free plasmid DNA in 15 minutes!
| Technical specifications | |||||||
| Applicable For | Eluted DNA is well suited for transfection, sequencing, cloning, plasmid mediated gene silencing, in vitro transcription, and other sensitive applications. | ||||||
| Binding Capacity | 1.2 mg | ||||||
| Culture Input | ≤ 25 mL | ||||||
| Elution Volume | ≥ 200 µl | ||||||
| Endotoxin Levels | ≤1 EU/µg Plasmid DNA | ||||||
| Equipment | Microcentrifuge and vacuum/vacuum manifold | ||||||
| Format | Spin Column | ||||||
| Processing Time | 15 min | ||||||
| Purity | Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0 | ||||||
| Recommended Sample Volume | ≤ 25 ml | ||||||
| Size Range | Up to 200 kb | ||||||
| Yield | ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized). 1.2 mg maximum binding capacity of column. | ||||||
A-105176 Disarticulated Half Child Skeleton Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
0091 Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
RE601A-W - Rotary EvaporatorRE601A-W - Yamato
D4074- Quick-DNA Microprep Plus Kit- Zymo Research
A-105867, Human Skull Model With Flexible Neck Axis Scientific
A-104272 Life-Size Human Skull With Blood Vessels Axis Scientific
0052 Snap On Tube Rack Cover, 96-Well, Polypropylene, Clear Natural Bio Plas
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
M3011- Dual Media Set 100 ml EB & 500 ml OB- Zymo Research
D4075- Quick-DNA Midiprep Plus Kit- Zymo Research 


