Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl), EndoZero, vaccine-grade, and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
| Technical specifications | |||||||
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, genome editing, and other sensitive applications. | ||||||
| Binding Capacity | 1.2 mg | ||||||
| Culture Input | ≤ 150 ml | ||||||
| Elution Volume | ≥ 200 µl | ||||||
| Endotoxin Levels | ≤ 0.025 EU/µg Plasmid DNA | ||||||
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge. | ||||||
| Format | Spin Column | ||||||
| Processing Time | 20 min | ||||||
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0 | ||||||
| Recommended Sample Volume | ≤ 150 ml | ||||||
| Size Range | Up to 200 kb | ||||||
| Yield | ≤ 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized) | ||||||
A-105171 Half Disarticulated Human Skeleton Axis Scientific
BS-7000B Inverted Fluorescent Biological Microscope BetScope
A-105177 Painted And Numbered 3-Part Life-Size Human Skull Axis Scientific
A-105759, Life-Size Human Skull With Removable Muscles Axis Scientific
A-105168 Miniature Human Skeleton Axis Scientific
A-104267 Classic Human Skeleton With Study & Numbering Guide Axis Scientific
Q620 WashLab760 Laboratory Bottle Washer Kimadi
A-105852 Classic Flexible Human Skeleton Axis Scientific
V1002-S8 Optional eight channel adapter, fine tip stainless steel Accuris
0096F WELL MICROCENTRIFUGE TUBE RACK, FLUORESCENT YELLOW, 5/PK Bio Plas
A-105939, 22 Part Osteopathic Didactic Human Skull Axis Scientific
9132010 10x objective NA 0.25/WD 6.64mm (LP Series DIN Phase Achromatic) Labomed
A-105867, Human Skull Model With Flexible Neck Axis Scientific
A-105940, 22 Part Osteopathic Natural Bone Human Skull Axis Scientific
A-105179, 22-Part Disarticulated Life-Size Human Skull Axis Scientific
A-104270, 3-Part Life-Size Numbered Human Skull Axis Scientific
A-104281 Painted Human Skeleton Axis Scientific
911015119999 D2012 Plus High Speed Personal Micro-Centrifuge Scilogex
A-105868, Human Skull With Masticatory Muscles Axis Scientific
0097 Lavender Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
A-104406 Complete Disarticulated Human Skeleton Axis Scientific
WashLab790 Laboratory Bottle Washer Kimadi
A-105176 Disarticulated Half Child Skeleton Axis Scientific
0095 Pink Polypropylene Reversible 1.5ml And 2.0ml Microcentrifuge Tube Rack, 96 Places (Pack Of 5) Bio Plas
R1056- Quick-RNA Midiprep Kit 25 preps- Zymo Research 



