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45-2040 Gemini Twin Wave Electroporators BTX


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Flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more.

$11,437.00

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The Gemini Twin Wave Electroporators are flexible systems allowing both square wave and exponential decay wave electroporation in a single unit. Ideal for all of your electroporation needs, including CRISPR, in vivo, in vitro, in ovo and more. Systems for cuvettes or plates.

These waveform combinations enable researchers to easily and efficiently electroporate eukaryotic and prokaryotic cells in cuvettes or plates with one easy-to-use setup. Prokaryotic cells typically respond well to exponential decay wave pulses and eukaryotic cells are transfected most efficiently with square wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their applications.

FEATURES & BENEFITS

  • Square wave and exponential decay waves power in a single unit.
  • Multiple pulsing capabilities in both square and exponential decay waveforms (X2 systems)
  • Large, easy-to-use touch screen interface
  • Universal electroporation – transfects cells in vitro, in vivo, in ovo and adherent forms
  • Preset protocols – includes the most common eukaryotic and prokaryotic cell types
  • User-defined protocols – unlimited ability to add and modify protocols
  • Safety – displays resistance measurements for each pulse with three layers of arc protection
  • Data management – stores logs of every pulse delivered for QC and troubleshooting

GEMINI SC

The Gemini SC system is designed specifically for cuvette-based in vitro transfections of eukaryotic or prokaryotic cells in suspension. With a wide range of pulsing parameters, advanced safety features as well as dozens of preset protocols, the Gemini SC is ideal for any lab requiring efficient cell transfection or transformation without the use of costly reagents.

GEMINI X2 AND GEMINI X2 HT

The Gemini X2 system provides the ultimate experimental flexibility. This one easy setup allows for transfections in cuvettes or high throughput plates, or the capability to utilize the wide variety of BTX specialty electrodes for specific applications. In addition, Gemini X2 electrical output specifications are wide-ranging, making it the most versatile electroporation system available today.

Electroporation of suspension cells can be achieved in cuvettes (X2) or 96-well plates (X2 HT) with an HT plate handler. Additionally, the Gemini X2 can be paired with BTX specialty electrodes to deliver gene and drugs in vivo, in utero, in ovo and to explant tissues as well as adherent cells.


GEMINI’S BROAD CAPABILITIES

Exponential Decay/Square Twin Waveforms

Allows researchers to select which type of waveform they would like to use to transfect eukaryotic cells or transform prokaryotic cells. Typically, eukaryotic cells respond best to square wave pulses while prokaryotic cell respond well to exponential decay wave pulses. Combining these two waveforms gives researchers total flexibility to achieve the highest efficiency for their application.

Simple User Interface
Permits intuitive touch screen or computer-controlled protocol programing and execution as well as password protection of protocols.Each system is programmable and offers unlimited protocol storage as well as dozens of proven pre-set protocols to reduce optimization time. The X2 systems provide protocol delivery log storage. Logs can be accessed for quality control, optimization or troubleshooting purposes.

Pre-Pulse Resistance Measurement
Offers assurance that sample resistance is within acceptable range for electroporation. This feature protects precious sample as well as system component to safeguard against arcing.

Widest Voltage/Pulse length Range Available
Provides researchers with the widest range of voltage options available in the market.

  • The Gemini X2 can achieve voltage delivery as low as 5 V and as high as 3000 V in both waveforms in addition to pulse lengths of 10 µs to 5 s, from delicate in ovo tissue to hearty gram positive bacteria.
  • The Gemini SC can achieve voltage delivery as low as 10 V and as high as 3000 V in both waveforms in addition to pulse lengths of 50 µs to 5 s.

Highly Accurate Pulsing
Delivers precise voltage, pulse length/time constant, pulse interval and displays the values back to the researcher allowing for visual confirmation of successful electroporation.

Revolutionary Arc Proofing Safety Features
Gives researchers peace of mind knowing that their sample and their systems are safe from troublesome arcing with enhanced 4-way safety checks through the pulsing procedure. Using two separate pre pulse, sample resistance monitoring scans to ensure proper conditions, as well as internal safety features auditing pulse progress in at the component as well as software level, arcing is dramatically decreased.

The Gemini is intended For Research Use Only.  Not for use in diagnostic, pre-clinical, or clinical procedures.

GEMINI X2 SPECIFICATIONS

SQUARE WAVE PULSE RANGES
Voltage Range
   LV Mode 5 to 500 V in 1 V steps
    HV Mode 505 to 3000 V in 5 V steps
Voltage Accuracy
   LV Mode 5%
   HV Mode 5%
Pulse Length Range
   LV Mode 10 μs to 999 μs in 1 μs steps
1 ms to 999 ms in 1 ms steps
   HV Mode 10 μs to 600 μs in 1 μs steps
Multiple Pulsing
   Single Samples 1 to 99 pulses per sample
   HT Plate Handler Mode 1 to 10 pulses per sample (up to 120 total)
   Pulse Interval 0.1 s to 10 s
Capacitance
   LV Mode 3775 μF
   HV Mode 85 μF
   Voltage Droop 20% Load <1 K Ω, 10% Load >1 K Ω
EXPONENTIAL DECAY PULSE RANGES
Voltage Range
   LV Mode 5 to 500 in 1 V steps
   HV Mode 505 to 3000 in 5 V steps
Voltage Accuracy
   LV Mode 5%
   HV Mode 5%
Time Constant Range
   LV Mode 1 ms to 5.158 s
   HV Mode 0.5 ms to 133.875 ms
Multiple Pulsing
   Multiple Pulsing 1 to 2 depending on device’s current amplitude limit
   Pulse Interval 5 to 30 s
Capacitance
   LV Mode 25 to 3275 μF in 25 μF steps
   HV Mode 10, 25, 35, 50, 60, 75, 85 μF
Available Internal Resistance Selections
   All Modes LV 25 to 1575 Ω in 25 Ω steps
HV 50 to1575 Ω in 25 Ω steps
SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE
   LV Mode PL ≤100 ms, load ≥8 to 9 Ω, PL >100 ms, load ≥100 Ω
   HV Mode Load ≥40 Ω
GEMINI SC SPECIFICATIONS
SQUARE WAVE PULSE RANGES
Voltage Range
   LV Mode 10 to 500 V in 5 V steps
   HV Mode 1510 to 3000 V in 10 V steps
Voltage Accuracy
   LV Mode 5%
   HV Mode 5%
Pulse Length Range
   LV Mode 0.05 ms to 10 ms in 0.05 ms steps
10 ms to 100 ms in 1 ms steps
   HV Mode 50 μs to 5 ms in 50 μs steps
Multiple Pulsing
   LV Mode 1 to 10 pulses per sample
   HV Mode 1 to 2 pulses per sample
   Pulse Interval 0.1 s to 10 s
Capacitance
   LV Mode 3775 μF
   HV Mode 85 μF
   Voltage Droop 20% Load <1 K Ω, 10% Load >1 K Ω
EXPONENTIAL DECAY PULSE RANGES
Voltage Range
   LV Mode 10 to 500 V in 5 V steps
   HV Mode 510 to 3000 V in 10 V steps
Voltage Accuracy
   LV Mode 5%
   HV Mode 5%
Time Constant Range
   LV Mode 1.25 ms to 3.275 s / 1.25 ms
   HV Mode 0.5 ms to 50 ms / 0.5 ms
Multiple Pulsing
   Multiple Pulsing No
   Pulse Interval N/A
Capacitance
   LV Mode 25 to 3275 μF in 25 μF steps
   HV Mode 10, 25 and 50 μF
Available Internal Resistance Selections
   All Modes 50 to 1000 Ω in 50 Ω steps
SAMPLE RESISTANCE (LOAD) EXPONENTIAL DECAY OR SQUARE
   LV Mode Load ≥10 Ω
   HV Mode 510 – 2500 V Load ≥20 Ω, 2500 – 3000 V, Load ≥600 Ω
OTHER SPECIFICATIONS (X2 AND SC)
Charging Time LV <7 s, HV <4 s
Monitoring Pulse Voltage, Width, Droop % and Sample Resistance
Display 7.0-in color display
Controls Touch Screen
Programmability Storage over 1,000 Protocols
Arc Control Yes
Interlock Switches Yes
Pre-Pulse Sample Resistance Check Yes
Pulse Over Current Protection Yes
PC Control X2: Yes; SC: No
PC Communications X2: Yes; SC: No
Log Report X2: Yes; SC: No
Remote Operation X2: Yes; SC: No
Foot Switch Control X2: Yes; SC: No
Power Ratings 35 W idle and 350 W pulsing
Input Voltage Ratings 100 to 240 VAC
CE, ETL Marking, CB Scheme, EuRoHS Yes
Dimensions (H x W x D) 12.75 x 11.25 x 8.5
Weight ~16 lb
Atmospheric Specifications
    Operating Temperature 4˚C to 40˚C (40˚F to 104˚F)
   Storage Temperature -10˚C to 70˚C (14˚F to 158˚F)
    Storage Humidity 20% to 80% RH, non condensing
Mode of Operation Continuous
Classification Class I
Pollution Degree I
Installation Category II
Regulatory Certifications CE, ETL (UL, CSA), WEEE, EU RoHS & CB Scheme
SKU: 45-2040 Gemini Twin Categories: , Tags: , , , Brand:
Weight 16 lbs
Dimensions 8.5 × 11.25 × 12.75 in
Product

45-2040(Gemini X2 Electroporation System Complete, for Cuvettes)