201203/201205/201207/201209-Taq DNA Polymerase- Qiagen

Taq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer that minimizes the need for optimization of PCR parameters, as well as Q-Solution, a novel additive that enables efficient amplification of “difficult” (e.g., GC rich) templates. In addition, CoralLoad PCR Buffer (containing two gel-tracking dyes) is also provided, enabling immediate loading of PCR products.

Performance

Taq DNA Polymerase outperformed kits tested from other suppliers and delivers robust PCR performance in a wide range of PCR conditions, without the need for time-consuming optimization (see figures ” Tolerance of different primer Tm Values” and ” Specific amplification of long PCR products”). Every lot of Taq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified from human genomic DNA (see figure ” Lot-to-lot reproducibility”). The unique formulation of QIAGEN PCR Buffer and CoralLoad PCR Buffer, also provided with the kit, enable highly specific PCR in a variety of PCR conditions with minimal optimization requirements (see figures ” Wide annealing-temperature window” and ” Tolerance to variable magnesium concentration”). In addition, CoralLoad PCR Buffer enables immediate loading of PCR products onto an agarose gel for even easier handling and faster results. Suboptimal PCR can be improved using Q-Solution, a PCR additive, also provided with the kit (see figure ” Amplification of difficult templates”).

Taq DNA Polymerase specifications

Concentration: 5 units/µl
Recombinant enzyme: Yes
Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTP
Extension rate: 2–4 kb/min at 72°C
Half-life: 10 min at 97°C; 60 min at 94°C
Amplification efficiency: ≥105 fold
5’–>3′ exonuclease activity: Yes
Extra A addition: Yes
3’–>5′ exonuclease activity: No
Contaminating nucleases: No
Contaminating RNases: No
Contaminating proteases: No
Self-priming activity: No

Procedure

Taq DNA Polymerase ensures highly specific PCR for a range of applications — with minimal optimization of PCR parameters. The streamlined, easy-to-follow protocol provided with the kit simplifies PCR setup. For added convenience and easier handling, CoralLoad PCR Buffer is provided. PCR products can be directly loaded onto a gel without the addition of a loading dye. To ensure success with GC-rich templates, the PCR enhancer Q-Solution is included.

Applications

Taq DNA Polymerase is used for standard and specialized applications, including:

• General PCR
• RT-PCR
• Screening
• PCR-based DNA fingerprinting (VNTR, STR, and RAPD)