The YeaStar RNA Kit provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans/, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1-1.5 ml of cultured cells using innovative Zymo-Spin column technology.
| Technical specifications | |||||||
| Applicable For | Isolated RNA is well suited for downstream applications such as Northern blotting, RT-PCR, Next-Generation Sequencing, Microarray, hybridization and other sensitive applications | ||||||
| Elution Volume | ≥ 60 µl DNase/RNase-Free Water | ||||||
| Equipment | Microcentrifuge & Heat Block/Bath | ||||||
| Processing Time | 30 minutes | ||||||
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107cells) | ||||||
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 | ||||||
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) | ||||||
| Size Range | ≥ 17 nt | ||||||
| Type | Total RNA | ||||||
| Yield | ≤ 25 µg of total RNA | ||||||
H38878-0010, DRY ICE MAKER; U.S. MODEL, 12 X 6½ X 5 IN. Bel-Art
F123602-PIPETMAN Classic P1000-Gilson
701221110- FreeZone 12 Liter -50C Console Freeze Dryer with Stoppering Tray Dryer- Labconco
F144071-PIPETMAN G Multichannel P12x20G, 2-20µL-Gilson
9126200 Binocular Observation Head Labomed
SF4300- Stat Fax 4300 (Chromate)- Awareness Technology
PAC078MU01-Anti-LAMa5 Rabbit Polyclonal Antibody- Cloude Clone 



