E COLI TEST, Reagent and Kits
T3019/T3020- Mix & Go! Competent Cells-Zymo 10B- Zymo Research
$142.00 – $544.00
The Mix & Go! E. coli strains are premade, chemically competent cells for simple and highly efficient DNA transformation. Mix & Go! E. coli cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Can be used for blue/white screening and is ideal for cDNA generation and library construction. | ||||||
| Genotype | F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara leu) 7697 galU galK rpsL nupG λ- | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA | ||||||
| Product | T3019 (Mix & Go! Competent Cells-Zymo 10B – 10 x 100 µl), T3020 (Mix & Go! Competent Cells-Zymo 10B – 96 x 50 µl) |
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