T3007/T3009/T3010- Mix & Go! Competent Cells – DH5 Alpha- Zymo Research
$142.00 – $544.00
The Mix & Go! Competent Cells – DH5 Alpha are premade E. coli DH5 alpha competent cells for simple and highly efficient DNA transformation. These DH5 alpha competent cells are made chemically competent by a method that completely eliminates the need for heat shocking and related procedures. For transformation, simply mix DNA with cells and then spread onto solid medium − Mix & Go! The premade Mix & Go! competent cells are highly efficient (> 108 transformants/µg pUC19) and can be used for cloning, sub-cloning, PCR fragment cloning, library construction, etc.
| Technical specifications | |||||||
| Additional Info | Insert stability due to recA1 mutation. Can be used for blue/white screening, accepts large plasmids due to deoR mutation. High plasmid yield due to endA1 mutation. | ||||||
| Genotype | F-Φ80lacZΔM15 Δ(lacZYA-argF)U169 deoR nupG recA1 endA1 hsdR17(rK- mK+) phoA glnV44 (supE44) thi-1 gyrA96 relA1,λ- | ||||||
| Processing Time | 20 Seconds | ||||||
| Product Storage | -70°C to -80°C | ||||||
| Transformation Efficiency | 108 – 109 transformants per µg of plasmid DNA | ||||||
| Product | T3007 (Mix & Go! Competent Cells – DH5 Alpha – 10 x 100 µl), T3009 (Mix & Go! Competent Cells – DH5 Alpha – 96 x 50 µl), T3010 (Mix & Go! Competent Cells – DH5 Alpha w/ 96-well PCR plates and Cover Foils – 96 x 50 µl) |
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