RR064B/RR064A-One-step PrimeScript RT-PCR kit for real-time RT-PCR- Takara
$65.00 – $1,336.00
Real-time RT-PCR is made easy with the One Step PrimeScript RT-PCR Kit (Perfect Real Time), a one-step real-time RT-PCR kit designed for real-time RT-PCR (qPCR) via probe-based qPCR assays such as 5′ nuclease assays1. In this 5′ nuclease assay real-time PCR (qPCR) kit, one-step real-time RT-PCR can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following PCR. The sensitivity provided by the enzymes in this kit enables detection of very small amounts of RNA, including RNA derived from viral, rare, or precious samples.
This kit uses PrimeScript RTase, a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase that is verified to have low levels of RNase H and which exhibits an excellent extension rate and quick, full-length cDNA synthesis, as well as Takara Ex Taq HS, a high efficiency hot start PCR enzyme optimized for this one-step real-time RT-PCR kit.
Overview
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Enables accurate and rapid analysis of viral RNA or RNA derived from rare or precious sources.
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Contains Takara Ex Taq HS, a high-efficiency, hot-start PCR enzyme, for PCR.
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Buffer system is real-time PCR optimized, increasing amplification efficiency and detection sensitivity.
More Information
Applications
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One-step real-time RT-PCR
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Probe-based qPCR assays such as 5′ nuclease assays and real-time PCR (qPCR) assays
| Cat. # | Product | Size | Description |
| RR064B | One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) | 500 Rxns |
The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR. Cat. # RR064B contains 5 of Cat. # RR064A. Please refer to Cat. # RR064A for complete product documentation and resources. |
| RR064A | One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) | 100 Rxns |
The One Step PrimeScript RT-PCR Kit (Perfect Real Time) is designed for one step, real-time reverse transcription PCR (RT-PCR) using probe detection. With this kit, all RT-PCR steps can be performed in a single tube. Therefore, it is not necessary to add additional reagents during the reaction, minimizing the risk of contamination and simplifying the workflow. Amplified products are monitored in real time without need for gel electrophoresis after PCR. This kit is suitable for detection of small amounts of RNA. This kit uses PrimeScript Reverse Transcriptase, which allows efficient and rapid cDNA synthesis, and Takara Ex Taq HS, which enables highly efficient hot start PCR. |
| Product | RR064B (One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) – 500 Rxns), RR064A (One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) – 100 Rxns) |
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