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R045B/R045A-PrimeSTAR Max DNA Polymerase—fast and high-fidelity PCR- Takara


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Advantage 2 Polymerase Mix is an optimized blend of PCR enzymes that is a proven balance of high yield and high fidelity (with 3X higher fidelity than regular Taq) for cDNA amplification and library construction. With an automatic hot-start feature, our Advantage 2 Polymerase Mix can readily amplify a wide range of DNA templates, including long templates up to 18 kb, and complex genomic DNA up to 6 kb. The Advantage 2 Polymerase Mix includes two optimized buffers. dNTPs need to be purchased separately.

PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any Takara Bio enzyme. The formulation is based on Takara’s unique, high-fidelity PrimeSTAR HS DNA Polymerase combined with an elongation factor to provide efficient priming and extension, which greatly reduces the time required for annealing and extension steps. As a result, PrimeSTAR Max DNA Polymerase can be used for exceptionally fast PCR. It is formulated as a 2X premix containing optimized buffer and dNTPs, which allows for quick preparation of reactions for high-throughput applications.

In addition, standardization of the extension step time makes PrimeSTAR Max DNA Polymerase suitable for reactions with excessive nucleic acids that would ordinarily be difficult to amplify due to non-specific binding. The superior processivity of PrimeSTAR Max DNA Polymerase prevents inhibition by excessive nucleic acids, resulting in a much higher success rate for PCR with minimal required optimization. The antibody-mediated hot-start feature provides improved specificity by preventing false initiation events during the reaction assembly.

Overview

  • Highest fidelity of any commercially available DNA polymerase
  • Fastest extension speed means less time required for PCR cycles
  • Convenient 2X premix includes buffer and dNTPs, allowing reaction assembly in less time
  • Antibody-mediated hot-start formulation
  • Proven performance as reported in peer-reviewed literature

More Information

Applications

  • Fast PCR for high-throughput studies
  • Cloning and expression studies
  • Structure/function studies
  • Evolutionary studies (e.g., SNP analyses)
Cat. # Product Size Description
R045B PrimeSTAR® Max DNA Polymerase 400 Rxns A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio’s high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.  Cat. # R045B contains 4 of Cat. # R045A. Please refer to Cat. # R045A for complete product documentation and resources.
R045A PrimeSTAR® Max DNA Polymerase 100 Rxns A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio’s high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition.
639282 High Fidelity PCR EcoDry™ Premix 24 Rxns A lyophilized PCR master mix based on Takara’s hot-start Advantage 2 Polymerase Mix. The premix contains all neceeary reagents for a high-yield and high-fidelity PCR. Please refer to User Manual for the composition. EcoDry Premix are packaged as individual 8-well tube strips with optically clear sealing caps. Simply reconstitute the mix by adding PCR-grade water along with primers and template.
639280 High Fidelity PCR EcoDry™ Premix 48 Rxns A lyophilized PCR master mix based on Takara’s hot-start Advantage 2 Polymerase Mix. The premix contains all neceeary reagents for a high yield and high-fidelity PCR. Please refer to User Manual for the composition. EcoDry Premix are packaged as individual 8-well tube strips with optically clear sealing caps. Simply reconstitute the mix by adding PCR-grade water along with primers and template. Cat. # 639280 contains 2 of Cat. # 639282. Please refer to Cat. # 639282 for complete product documentation and resources.
SKU: R045B/R045A Categories: , Tags: , Brand:
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R045B (PrimeSTAR® Max DNA Polymerase – 400 Rxns), R045A (PrimeSTAR® Max DNA Polymerase – 100 Rxns)