Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
| Technical specifications | |||||||
| Concentration | 0.1 U/µl | ||||||
| Inactivation | 5X MN Stop Buffer or EDTA | ||||||
| Standard Reaction Time | 20 minutes | ||||||
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤-70°C. | ||||||
| Unit Definition | One unit (U) is defined as the amount of enzyme needed to produce an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2 (Kunitz, 1950). | ||||||
