dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
| Technical specifications | |||||||
| Concentration | 1 U/µl | ||||||
| Enzyme Inactivation | Heat inactivate enzyme at 65°C for 5 min. | ||||||
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C. | ||||||
| Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42°C in total reaction volume in 10 µl. | ||||||
| Product | E2018-50 (dsDNA Shearase Plus – 50 U), E2018-200 (dsDNA Shearase Plus – 200 U) |
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