Kits for Epigenetics, Reagent and Kits
D5310/D5311- OneStep qMethyl-PCR Kit- Zymo Research
$366.00 – $388.00
The OneStep qMethyl Kit provides a simple, straightforward, and bisulfite-free procedure for rapid, locus-specific DNA methylation assessment via the selective amplification of a methylated region of DNA. This is accomplished by splitting any DNA to be tested into two parts: a “Test Reaction” and a “Reference Reaction”. DNA in the Test Reaction is digested with Methylation Sensitive Restriction Enzymes (MSREs) while DNA in the Reference Reaction is not. The DNA from both samples is then amplified using real-time PCR in the presence of SYTO 9 fluorescent dye and quantitated using a simple equation.
| Technical specifications | |||||||
| Input | High quality DNA suitable for use in restriction enzyme digestion. Require A260/280 g&t; 1.8, A260/230 > 1.8. | ||||||
| Processing Time | 4.5 hours | ||||||
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze-thaw cycles of reagents. Prolong storage at -80°C. 2X Test Reaction PreMix and 2X Reference Reaction PreMix are light sensitive. Minimize light exposure. | ||||||
| Product | D5310 (OneStep qMethyl-PCR Kit – Full Kit), D5311 (OneStep qMethyl-Lite – Lite) |
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