Micrococcal Nuclease cleaves single-stranded and double-stranded DNA and RNA. Complete digestion with Micrococcal Nuclease yields mono- and oligonucleotides with 3′-phosphates.
| Technical specifications | |||||||
| Concentration | 0.1 U/µl | ||||||
| Enzyme Commission Number | (E.C. 3.1.31.1) | ||||||
| Enzyme Inactivation | EDTA or EGTA in molar excess of CaCl2. | ||||||
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing. Prolonged storage should be ≤ -70 °C. | ||||||
| Unit Definition | One unit (U) will produce 1.0 µmole of acid soluble polynucleotides from native DNA per min at pH 8.8 at 37°C, based on EM/260 = 10,000 for the mixed nucleotides. | ||||||
