Kits for Epigenetics, Reagent and Kits
D5030T/D5030/D5031- EZ DNA Methylation-Lightning Kit- Zymo Research
$50.00 – $602.00
The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.
| Technical specifications | |||||||
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. | ||||||
| Conversion | > 99.5% | ||||||
| Elution Volume | ≥ 10 µl | ||||||
| Equipment | Thermocycler with heated lid and microcentrifuge. | ||||||
| Input | 100 pg – 2 µg of DNA. | ||||||
| Processing Time | 1.5 hours | ||||||
| Recovery | > 80% | ||||||
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. | ||||||
| Product | D5030T (EZ DNA Methylation-Lightning Kit – 10 Rxns), D5030 (EZ DNA Methylation-Lightning Kit – 50 Rxns), D5031 (EZ DNA Methylation-Lightning Kit – 200 Rxns) |
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