Kits for Epigenetics, Reagent and Kits
D5023/D5022- EZ-96 DNA Methylation-Direct Kit- Zymo Research
$487.00
The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this 96-well bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
| Technical specifications | |||||||
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. | ||||||
| Conversion | > 99.5% | ||||||
| Elution Volume | Shallow well: > 30 µl Deep-well: >15 µl |
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| Equipment | Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers. | ||||||
| Input | Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105cells. | ||||||
| Processing Time | 4 hours | ||||||
| Recovery | > 80% | ||||||
| Sample Source | Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. | ||||||
| Product | D5023 (EZ-96 DNA Methylation-Direct Kit (Deep-well) – 2 x 96 Rxns.), D5022 (EZ-96 DNA Methylation-Direct Kit (Shallow- well) – 2 x 96 Rxns) |
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