D4300/D4300T/D4304- ZymoBIOMICS DNA Miniprep Kit- Zymo Research
$44.00 – $302.00
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
| Technical specifications | |||||||
| Applicable For | All sensitive downstream applications such as qPCR and Next-Generation Sequencing. | ||||||
| Elution Volume | ≥ 50 µl DNA Elution Buffer or DNase free water | ||||||
| Equipment | Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). | ||||||
| Processing Volume | Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2×109bacterial, 2×108yeast, or 2×107mammalian cells) | ||||||
| Purity | High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 | ||||||
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms, and water. | ||||||
| Sample Storage | Eluted DNA should be stored at ≤ -20°C | ||||||
| Size Range | Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. | ||||||
| Type | Total DNA | ||||||
| Yield | Up to 25 µg total DNA can be eluted into ≥50 µl | ||||||
| Product | D4300 (ZymoBIOMICS DNA Miniprep Kit (Lysis Tubes) – 50 Preps), D4300T (ZymoBIOMICS DNA Miniprep Kit – 5 Preps), D4304 (ZymoBIOMICS DNA Miniprep Kit (No Lysis Matrix) – 50 Preps) |
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